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IRCCS Ospedale Policlinico San Martino
Interlab Cell Line Collection (ICLC)
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Cell line namePF97387
Accession numberICLC HTL14004
Brief descriptionSpecies: human; Tissue: lymphocyte, B; Transformed by: Epstein-Barr
DescriptionSpecies: human male; Tissue: lymphocyte, B; Transformed by: Epstein-Barr
DepositorDr. M. P. Pistillo, IRCCS AOU San Martino - IST, Genoa, Italy
Reference paperTissue Antigens. 1996 Jun;47(6):538-46 - PMID: 881374
Morphology and growthcontinuous culture, grown in suspension, morphology lymphoblast like
Culture conditionsRPMI 1640 + 10% FBS + 2mM L-Glutamine; Split 1:2-1:3 twice weekly. 37C, 5% CO2
DistributionCell line available for distribution. For non-commercial investigative use only
HazardHandle under Biosafety Level 2 containment
Passage number> 20
Freezing medium95% FCS + 5% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
HLA typing: A29; B44; Bw4; DR4; DRw53; DQ7. A*2902; Cw*1603; B*44031; DRB1*04; DQA1*03; DQB1*0301; DPA1*01; DPB1*0401; DPB1*0402; MICA*004
CommentsOriginator of cell line: Prof. R. Fauchet. Cell line from the 10th International Histocompatibility Workshop Cell Panel (10IHW).

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