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IRCCS Ospedale Policlinico San Martino
Interlab Cell Line Collection (ICLC)
On-Line Catalogue

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Cell line nameAMALA
Accession numberICLC HTL14002
Brief descriptionSpecies: human; Tissue: lymphocyte, B; Transformed by: Epstein-Barr
DescriptionSpecies: human female; Tissue: lymphocyte, B; Transformed by: Epstein-Barr
DepositorDr. M. P. Pistillo, IRCCS AOU San Martino - IST, Genoa, Italy
Reference paperTissue Antigens. 1996 Jun;47(6):538-46. - DOI: 10.1111/j.1399-0039.1996.tb02596.x - PMID: 8813743
Morphology and growthcontinuous culture, grown in suspension, morphology lymphoblast like
Culture conditionsRPMI 1640 + 10% FBS + 2mM L-Glutamine; Split 1:2-1:3 twice weekly. 37C, 5% CO2
DistributionCell line available for distribution. For non-commercial investigative use only
HazardHandle under Biosafety Level 2 containment
Passage number> 20
Freezing medium95% FCS + 5% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
HLA typing: A2; Cw9; B62; Bw6; DR14; DRw52; DQ7. A*2171; Cw*0303; B*1501; DRA*0102; DRB1*01402; DRB3*0101; DQA1*0503; DQB1*03011; DPA1*01; DPB1*0402
CommentsOriginator of cell line: Prof. Z. Layrisse. Cell line from the 10th International Histocompatibility Workshop Cell Panel (10IHW).

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