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IRCCS Ospedale Policlinico San Martino
Interlab Cell Line Collection (ICLC)
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Cell line nameUPMM 3
Accession numberICLC HTL13001
Brief descriptionSpecies: human; Tissue: uvea; Tumor: melanoma, uveal
DescriptionSpecies: human male; Tissue: uvea; Tumor: melanoma, uveal
DepositorDr. U. Pfeffer, IRCCS AOU San Martino - IST, Genoa, Italy
Reference paperOphthalmologica. 2009;223(3):196-201 - DOI: 10.1159/000201566 - PMID: 19212147
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial
Culture conditionsRPMI 1640 + 20% FBS + 2mM L-Glutamine; 37°C, 5% CO2, split cultures 1:2-1:4
PropertiesMelanin production
DistributionCell line available for distribution. For non-commercial investigative use only
Freezing mediumCulture medium 45% + 50% FCS + 5% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
D5S818: 10,11; D13S317: 8,11; D7S820: 8,11; D16S539: 12; VWA: 16,17; TH01: 7,9.3; AM: X,Y; TPOX: 9,10; CSF1PO: 12,13; D21S11: 28,31.2
CommentsEstablished from the primary tumor of a man with uveal melanoma. Single cells, slow and regular growth.

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