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IRCCS Ospedale Policlinico San Martino
Interlab Cell Line Collection (ICLC)
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Cell line nameMEL 290
Accession numberICLC HTL12002
Brief descriptionSpecies: human; Tissue: uvea; Tumor: melanoma, uveal
DescriptionSpecies: human female; Tissue: uvea; Tumor: melanoma, uveal
DepositorDr. U. Pfeffer, IRCCS AOU San Martino - IST, Genoa, Italy
Reference paperUnknown
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial
Culture conditionsRPMI 1640 + 20% FBS + 2mM L-Glutamine; 37C, 5% CO2, split confluent cultures 1:3-1:4 using trypsin/EDTA
PropertiesMinimal melanin production
DistributionCell line available for distribution. For non-commercial investigative use only
Freezing mediumCulture medium 45% + 50% FCS + 5% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
D5S818: 10,12; D13S317: 8,11; D7S820: 11,12; D16S539: 11,13; VWA: 14,16; TH01: 6,9; AM: X; TPOX: 8,11; CSF1PO: 10,11; D21S11: 28,32.1
CommentsEstablished from the primary tumor of a woman with uveal melanoma. Single cells, slow and regular growth.

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