Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell line name | MDA-MB-436 |
Accession number | ICLC HTL11005 |
Brief description | Species: human, Caucasian; Tissue: mammary gland; Tumor: adenocarcinoma, breast |
Description | Species: human, Caucasian female 43 years old; Tissue: mammary gland; Tumor: adenocarcinoma, breast; Derived from: pleural effusion |
Depositor | Dr. A. Garuti, Cell Therapy, DIMI, Genoa, Italy |
Reference paper | Cancer Res 1979;39: 919-922 - PMID: 427779 |
Morphology and growth | continuous culture, grown as monolayer, morphology pleomorphic with multinucleated component cells |
Culture conditions | RPMI 1640 + 10% FBS + 2mM L-Glutamine; 37C, 5% CO2, split cultures 1:2-1:5 using trypsin/EDTA |
Distribution | Cell line available for distribution. For non-commercial investigative use only |
Hazard | |
Passage number | 8 |
Freezing medium | Culture medium 45% + 50% FCS + 5% DMSO |
Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |