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IRCCS Ospedale Policlinico San Martino
Interlab Cell Line Collection (ICLC)
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Cell line nameU-2 OS
Accession numberICLC HTL11001
Brief descriptionSpecies: human, Caucasian; Tissue: bone; Tumor: osteosarcoma
DescriptionSpecies: human, Caucasian female 15 years old; Tissue: bone; Tumor: osteosarcoma; Derived from: sarcoma of the tibia of a 15 year old girl
DepositorDr. R. Cancedda, IRCCS AOU San Martino - IST, Genoa, Italy
Reference paperCancer Res 1997;57: 3562-3568 - PMID: 9270029
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine; 37C, 5% CO2, split confluent cultures 1:3-1:6 using trypsin/EDTA
DistributionCell line available for distribution upon consent of the depositor. For non-commercial investigative use only
Freezing medium95% FCS + 5% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.

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