Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell line name | U-2 OS |
Accession number | ICLC HTL11001 |
Brief description | Species: human, Caucasian; Tissue: bone; Tumor: osteosarcoma |
Description | Species: human, Caucasian female 15 years old; Tissue: bone; Tumor: osteosarcoma; Derived from: sarcoma of the tibia of a 15 year old girl |
Depositor | Dr. R. Cancedda, IRCCS AOU San Martino - IST, Genoa, Italy |
Reference paper | Cancer Res 1997;57: 3562-3568 - PMID: 9270029 |
Morphology and growth | continuous culture, grown as monolayer, morphology epithelial |
Culture conditions | DMEM + 10% FBS + 2mM L-Glutamine; 37C, 5% CO2, split confluent cultures 1:3-1:6 using trypsin/EDTA |
Distribution | Cell line available for distribution upon consent of the depositor. For non-commercial investigative use only |
Hazard | |
Freezing medium | 95% FCS + 5% DMSO |
Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |