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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | SK-HEP-1 |
| Accession number | ICLC HTL10001 |
| Brief description | Species: human, Caucasian; Tissue: liver; Tumor: adenocarcinoma |
| Description | Species: human, Caucasian male; Tissue: liver; Tumor: adenocarcinoma |
| Depositor | A M Bassi e S Penco, DIMES, General Pathology, Cell Cultures Lab, University of Genoa, Italy |
| Reference paper | Unknown |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial-like |
| Culture conditions | MEM (EBSS) + 10% FBS + 1% non-essential amino acids; split confluent cultures 1:5 - 1:10 using trypsin/EDTA, 37C, 5% CO2 |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Freezing medium | Culture medium 45% + 50% FCS + 5% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. Multiplex PCR: tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers |