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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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| Cell line name | U87/WT |
| Accession number | ICLC HTL08004 |
| Brief description | Species: human; Tissue: brain; Tumor: glioblastoma; Transfected with: Transfected with wt EGFR |
| Description | Species: human; Tissue: brain; Tumor: glioblastoma; Transfected with: Transfected with wt EGFR |
| Depositor | Dr T. Florio, DOBIG, Pharmacology Dept, University of Genoa, Italy, |
| Reference paper | Proc Natl Acad Sci U S A. 1994 Aug 2;91(16):7727-31 - DOI: 10.1073/pnas.91.16.7727 - PMID: 8052651 |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial-like |
| Culture conditions | DMEM + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:6 using trypsin/EDTA; 37C, 5% CO2 |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | Handle as Biosafety Level 2 containment |
| Freezing medium | Culture medium 45% + 50% FCS + 5% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. Multiplex PCR: tested again human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers |