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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | A2058 |
| Accession number | ICLC HTL08002 |
| Brief description | Species: human, Caucasian; Tissue: skin; Tumor: melanoma, metastatic |
| Description | Species: human, Caucasian male 43 years old; Tissue: skin; Tumor: melanoma, metastatic; Derived from: lymph node metastasis |
| Depositor | Dr F. Tosetti, Cellular Biology Dept, IST at ABC, Genoa, Italy |
| Reference paper | Unknown |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial |
| Culture conditions | RPMI 1640 + 10% FBS + 2mM-Glutamine + 4,5 g/L glucose; split confluent cultures1:3-1:6; 37C, 5% CO2 |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Freezing medium | Culture medium 45% + 50% FCS + 5% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. Multiplex PCR: tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers |
| Further bibliography | Proc Natl Acad Sci U S A. 1977;74(2):565-9 - DOI: 10.1073/pnas.74.2.565
- PMID: 265522
Proc Natl Acad Sci U S A. 1979; 76(3):1288-92 - DOI: 10.1073/pnas.76.3.1288 - PMID: 375235 Proc Natl Acad Sci U S A. 1980;77(9):5258-62 - DOI: 10.1073/pnas.77.9.5258 - PMID: 6254071 |