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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | MeMo 05 |
| Accession number | ICLC HTL07002 |
| Brief description | Species: human; Tissue: lymph node, metastasis; Tumor: melanoma |
| Description | Species: human male 66 years old; Tissue: lymph node, metastasis; Tumor: melanoma |
| Depositor | Dr G. Ferlazzo, Human Pathology Dept., Pad-H, University of Messina, Italy |
| Reference paper | Unknown |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial |
| Culture conditions | RPMI 1640 + 10% FBS + 2mM L-Glutamine; 37C, 5% CO2, split confluent cultures 1:3 - 1:4 using trypsin/EDTA |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | Handle under Biosafety Level 2 containment |
| Passage number | 19 |
| Freezing medium | Culture medium 45% + 50% FCS + 5% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. Multiplex PCR: tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers |
| Comments | Originator of cell line: Dr P. Carrega |