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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | CM-S/un |
| Accession number | ICLC HTL06005 |
| Brief description | Species: human, Caucasian; Tissue: bone marrow; Tumor: monocyte tumor |
| Description | Species: human, Caucasian; Tissue: bone marrow; Tumor: monocyte tumor; Derived from: patient with defects of haematopoiesis |
| Depositor | Dr. R. P. Revoltella, Fondazione ONLUS - Staminali e Vita - Pisa, Italy |
| Reference paper | Cancer Res 1982;42(10):4182-9 - PMID: 7105012 |
| Morphology and growth | continuous culture, grown in suspension, morphology monocytic, round, single and small clusters |
| Culture conditions | RPMI 1640 + 10% FBS + 2mM L-Glutamine; split 1:10-1:15, 37C, 5% CO2 |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Passage number | 4 |
| Freezing medium | Culture medium 50% + 40%FCS + 10%DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. Multiplex PCR: tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers |
| Further bibliography | Cancer Res. 1985;45(3):1392-401 - PMID: 3971383
FEBS Lett. 1985 Sep 9;189(1):97-101 - DOI: 10.1016/0014-5793(85)80850-4 - PMID: 2993032 |
| Comments | Thawed with 20%FCS. |