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Cell line nameNCI-H1975
Accession numberICLC HTL06001
Brief descriptionSpecies: human; Tissue: lung; Tumor: adenocarcinoma, non-small cell
DescriptionSpecies: human female; Tissue: lung; Tumor: adenocarcinoma, non-small cell
DepositorDr P. Russo, Translational Oncology Dept, IST at ABC, Genoa, Italy
Reference paperScience 2004;305(5687):1163-7 - DOI: 10.1126/science.1101637 - PMID: 15284455
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like
Culture conditionsRPMI 1640 + 2 mM L-Glutamine + 10% FBS + 1mM Na pyruvate; split confluent cultures1:3-1:6, 37C, 5% CO2
DistributionCell line available for distribution. For non-commercial investigative use only.
Freezing mediumCulture medium + 5% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Multiplex PCR: tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers
Further bibliographyJ Cell Biochem Suppl 1996;24:1-291 - PMID: 8965489
CommentsCell line from a non-smoker patient

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