Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell line name | THP-1 l |
Accession number | ICLC HTL05009 |
Brief description | Species: human; Tissue: peripheral blood; Tumor: leukemia, acute monocytic |
Description | Species: human; Tissue: peripheral blood; Tumor: leukemia, acute monocytic; Derived from: THP-1 |
Depositor | Dr A. De Lerma, Clinical and Biological Science Dept, Faculty of Medicine, Insubria University, Italy |
Reference paper | Unknown |
Morphology and growth | continuous culture, grown in suspension, morphology large, round, single cells |
Culture conditions | RPMI 1640 + 10% FBS + 2mM L-Glutamine; Maintain cultures between 2-9x105 cells/ml; 37C, 5% CO2 |
Properties | No constitutive expression of HLA-II; very low or null expression of HLA-II after treatment with IFNgamma |
Distribution | Cell line available for distribution. For non-commercial investigative use only |
Hazard | |
Freezing medium | 90% FBS + 10% DMSO |
Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. Amelogenin: X,Y; vWA: 16; FGA: 24,25; TH01: 8,9.3; D18S51: 13,14; D21S11: 30,31.2; D8S1179: 10,14 |