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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | H9 |
| Accession number | ICLC HTL05006 |
| Brief description | Species: human, Caucasian; Tissue: lymphocyte, T; Tumor: lymphoma |
| Description | Species: human, Caucasian male 53 years old; Tissue: lymphocyte, T; Tumor: lymphoma; Derived from: HuT 78 |
| Depositor | Dr. A. De Maria, Internal Medicine Dept (DIMI), Genoa, Italy |
| Reference paper | AIDS Res Hum Retroviruses 1989; 5(3):253-5 - DOI: 10.1089/aid.1989.5.253 - PMID: 2567177 |
| Morphology and growth | continuous culture, grown in suspension, morphology lymphoblast-like, cells growing single and in clusters |
| Culture conditions | RPMI 1640 + 10% FBS + Antibiotics + 2mM L-Glutamine; split 1:10 every 2 days; 37C, 5% CO2 |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Freezing medium | 90% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. Multiplex PCR: tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers |
| Further bibliography | Blood 1980; 55:409-417 - PMID: 6244013
J Natl Cancer Inst 1992; 84:1922-1926 - DOI: 10.1093/jnci/84.24.1922 - PMID: 1460674 |
| Comments | Thaw the cells with medium supplemented with FBS 20% |