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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | LB-F9 |
| Accession number | ICLC HTL05004 |
| Brief description | Species: human; Tissue: lymphocyte, B; Pathology: paroxysmal nocturnal hemoglobinuria; Transformed by: Epstein-Barr |
| Description | Species: human female; Tissue: lymphocyte, B; Pathology: paroxysmal nocturnal hemoglobinuria; Transformed by: Epstein-Barr |
| Depositor | Dr R. Notaro, Human Genetic Dept IST, Genoa, Italy |
| Reference paper | Unknown |
| Morphology and growth | continuous culture, grown in suspension, morphology lymphoblast, single and in small clusters growth |
| Culture conditions | RPMI 1640 + 4 mML-Glutamine + 20% FBS ; maintain cultures between 350-400 x 10^3 cells/ml; duplication time 48-60 h; 37C, 5% CO2 |
| Properties | GPI- |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | Handle under Biosafety Level 2 containment |
| Freezing medium | 90% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. Multiplex PCR: tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers |