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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | ROV-S |
| Accession number | ICLC HTL05002 |
| Brief description | Species: human, Caucasian; Tissue: bone marrow; Transformed by: Epstein-Barr |
| Description | Species: human, Caucasian; Tissue: bone marrow; Transformed by: Epstein-Barr; Derived from: children with maturation defects of haematopoiesis |
| Depositor | Dr. R. P. Revoltella, Fondazione ONLUS - Staminali e Vita - Pisa, Italy |
| Reference paper | Unknown |
| Morphology and growth | continuous culture, grown in suspension, morphology monocytic, round, single and small clusters |
| Culture conditions | RPMI 1640 + 20%FCS + 5ng/ml GM-CSF + 2mML-Glutamine; split 1:5, 37C, 5% CO2 |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Passage number | 51 |
| Freezing medium | Culture medium 50% + 40%FCS + 10%DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. Multiplex PCR: tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers |
| Further bibliography | J Gen Virol 1989;70:(5)1203-15 - DOI: 10.1099/0022-1317-70-5-1203
- PMID: 2543775
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