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Cell line nameTF-1
Accession numberICLC HTL05001
Brief descriptionSpecies: human; Tissue: bone marrow; Tumor: erythroleukemia
DescriptionSpecies: human male 35 years old; Tissue: bone marrow; Tumor: erythroleukemia
DepositorDr R. P. Revoltella, Istituto di Tecnologie Biomediche (ITB), CNR, Pisa, Italy
Reference paperUnknown
Morphology and growthcontinuous culture, grown in suspension, morphology erythroblastoid, round, single and some small clusters.
Culture conditionsRPMI 1640 + 20%FCS + 5ng/ml GM-CSF + 2mML-Glutamine; split 1:10, 37C, 5% CO2
DistributionCell line available for distribution. For non-commercial investigative use only
Passage number10
Freezing mediumCulture medium 50% + 40%FCS + 10%DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Multiplex PCR: tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers
Further bibliographyInt Immunol 1990;2(7):669-675 - DOI: 10.1093/intimm/2.7.669 - PMID: 2278997

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