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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | TF-1 |
| Accession number | ICLC HTL05001 |
| Brief description | Species: human; Tissue: bone marrow; Tumor: erythroleukemia |
| Description | Species: human male 35 years old; Tissue: bone marrow; Tumor: erythroleukemia |
| Depositor | Dr R. P. Revoltella, Istituto di Tecnologie Biomediche (ITB), CNR, Pisa, Italy |
| Reference paper | Unknown |
| Morphology and growth | continuous culture, grown in suspension, morphology erythroblastoid, round, single and some small clusters. |
| Culture conditions | RPMI 1640 + 20%FCS + 5ng/ml GM-CSF + 2mML-Glutamine; split 1:10, 37C, 5% CO2 |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Passage number | 10 |
| Freezing medium | Culture medium 50% + 40%FCS + 10%DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. Multiplex PCR: tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers |
| Further bibliography | Int Immunol 1990;2(7):669-675 - DOI: 10.1093/intimm/2.7.669
- PMID: 2278997
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