Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
|
Home - Search by name - Free text search - Search by properties |
Cell line name | A-498 |
Accession number | ICLC HTL04005 |
Brief description | Species: human; Tissue: kidney; Tumor: adenocarcinoma |
Description | Species: human female 52 years old; Tissue: kidney; Tumor: adenocarcinoma |
Depositor | Dr. M. Viale, Pharmacology Dept, IST, Genoa, Italy. |
Reference paper | Unknown |
Morphology and growth | continuous culture, grown as monolayer, morphology epithelial |
Culture conditions | MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1mM Na pyruvate; Split confluent cultures 1:3-1:6 three times a week using trypsin/EDTA (2-3 minutes); 37C, 5% CO2 |
Distribution | Cell line available for distribution. For non-commercial investigative use only |
Hazard | |
Freezing medium | Culture medium + 50% FBS + 10% DMSO |
Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
Further bibliography | J Natl Cancer Inst 1977;58:209-214 - DOI: 10.1093/jnci/58.2.209
- PMID: 833871
J Natl Cancer Inst 1977;59:221-226 - DOI: 10.1093/jnci/59.1.221 - PMID: 327080 |