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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | HOS |
| Accession number | ICLC HTL04003 |
| Brief description | Species: human, Caucasian; Tissue: bone; Tumor: osteosarcoma |
| Description | Species: human, Caucasian female 13 year old; Tissue: bone; Tumor: osteosarcoma |
| Depositor | Prof. A. Gasperi-Campani, Sperimental Pathology Dept, University of Bologna, Italy |
| Reference paper | Cancer 1971;27:397-402 - DOI: 10.1002/1097-0142(197102)27:2<397::aid-cncr2820270224>3.0.co;2-x - PMID: 5100401 |
| Morphology and growth | continuous culture, grown as monolayer, morphology fibroblast-like and epithelial-like |
| Culture conditions | DMEM + 10% FBS + 2mM L-Glutamine; Split sub-confluent (70-80%) cultures 1:10-1:12 twice for week using trypsin/EDTA; 37°C, 5% CO2 |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Passage number | 48 |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. Multiplex PCR: tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers |
| Further bibliography | Int J Cancer 1975;15:23-29 - DOI: 10.1002/ijc.2910150104
- PMID: 165148
Int J Cancer 1975;16:840-849 - DOI: 10.1192/bjp.131.6.610 - PMID: 23195 |