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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | M-07e |
| Accession number | ICLC HTL04002 |
| Brief description | Species: human, Caucasian; Tissue: peripheral blood; Tumor: leukemia, acute megakaryoblastic |
| Description | Species: human, Caucasian female 6 month old; Tissue: peripheral blood; Tumor: leukemia, acute megakaryoblastic; Derived from: M-07 growth factor-independent cell line |
| Depositor | Dr L. Pegoraro, University of Torino, Italy |
| Reference paper | Unknown |
| Morphology and growth | continuous culture, grown in suspension, morphology single round cells and some small clusters, few cells are lightly adherent |
| Culture conditions | RPMI 1640 + 20% FBS + 2mM L-Glutamine + 10 ng/ml GM-CSF; Slit 1:2-1:3 every two days; 37C, 5% CO2 |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Freezing medium | 90% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. Multiplex PCR: tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers |
| Further bibliography | Brit J Haematol 1988;69:359-366 - DOI: 10.1111/j.1365-2141.1988.tb02374.x
- PMID: 3261598
J Cell Physiol 1990;145:458-464 - DOI: 10.1002/jcp.1041450310 - PMID: 2273055 Leukemia 1997;11:701-708 - DOI: 10.1038/sj.leu.2400633 - PMID: 9180295 |
| Comments | The viability may be low after thawing and need about one week to recover. The cells grow also with Iscove's MDM medium instead of RPMI and with IL-3 (10 ng/ml) instead of GM-CSF. |