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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | 293T |
| Accession number | ICLC HTL04001 |
| Brief description | Species: human; Tissue: kidney, embryonic; Transformed by: SV40large T antigen; Original line: 293 |
| Description | Species: human; Tissue: kidney, embryonic; Transformed by: SV40large T antigen; Original line: 293 |
| Depositor | Dr R. Notaro, Human Genetics Dept IST, Genoa, Italy |
| Reference paper | Unknown |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial-like |
| Culture conditions | DMEM + 20% FBS + 2mM L-Glutamine; Split subconfluent cultures (70% confluence)1:4-1:8 using trypsin/EDTA; 37°C, 5% CO2 |
| Properties | SV40 large T antigen expression. Used for transfection assays and as packaging cells. The cell line can be transiently transfected and give extremely high levels of expression of AP fusion proteins. G418 resistant, neomicine resistant. The cell line is known also as 293tsA1609neo. |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | Handle under Biosafety Level 2 containment |
| Freezing medium | 90% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. Multiplex PCR: tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers |
| Further bibliography | Mol Cell Biol 1987;7:379-387 - DOI: 10.1128/mcb.7.1.379-387.1987
- PMID: 3031469
Proc Natl Acad Sci USA 1993;90:8392-8396 - DOI: 10.1073/pnas.90.18.8392 - PMID: 7690960 |
| Comments | The cells detach easily with PBS, but trypsin is necessary to disaggregate the cells |