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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | SK-BR-3 |
| Accession number | ICLC HTL03005 |
| Brief description | Species: human; Tissue: breast; Tumor: adenocarcinoma |
| Description | Species: human female 43 year old; Tissue: breast; Tumor: adenocarcinoma; Derived from: metastatic site: pleural effusion |
| Depositor | Prof. L. Lanfrancone, Experimental Oncology Dept, European Institute of Oncology, Milan, Italy |
| Reference paper | Unknown |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial, some round loosely attached cells |
| Culture conditions | DMEM + 10% FBS + 2mM L-Glutamine; Split 1:2-1:4 using trypsin/EDTA; 37C, 5% CO2 |
| Properties | The cell line overexpresses the HER/2c-erb-2 gene product |
| Distribution | Cell line available for distribution. For non-commercial investigative use only. |
| Hazard | |
| Freezing medium | 90% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. Multiplex PCR: tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers |
| Further bibliography | J Natl Cancer Inst 1977;59:221-226 - DOI: 10.1093/jnci/59.1.221
- PMID: 327080
Recent Results Cancer Res 1976;57:33-41 - DOI: 10.1007/978-3-642-81043-5_5 - PMID: 1013510 |