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Cell line nameB1647
Accession numberICLC HTL03004
Brief descriptionSpecies: human; Tissue: bone marrow; Tumor: leukemia
DescriptionSpecies: human male 14 year old; Tissue: bone marrow; Tumor: leukemia; Derived from: a patient with acute myeloid leukemia with a complete erythro-megakaryocytic phenotype
DepositorProf. G.P. Bagnara, Institute of Histology and Embryology, University of Bologna, Italy
Reference paperBr J Haematol 1997;98:549-559 - DOI: 10.1046/j.1365-2141.1997.2793096.x - PMID: 9332307
Morphology and growthcontinuous culture, grown in suspension, morphology round cells, erythromegakaryocytic
Culture conditionsIscove's modified Dulbecco's medium + 10% FBS + 2mM L-Glutamine ; Split 1:3-1:6; 37C, 5% CO2
PropertiesExpression of both c-mpl and erythropoietin receptor (Epo-R). Spontaneous B1647 cell proliferation is significantly potentiated by thrombopoietin (TPO)
DistributionCell line available for distribution upon consent of the depositor. For non-commercial investigative use only
Passage number>50
Freezing mediumCulture medium + 70% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Multiplex PCR: tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers
Further bibliographyHaematologica 2002;87:1242-1247 - PMID: 12495897

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