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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | HEL 92.1.7 |
| Accession number | ICLC HTL03002 |
| Brief description | Species: human, Caucasian; Tumor: erythroleukemia |
| Description | Species: human, Caucasian male 30 year old; Tumor: erythroleukemia |
| Depositor | Obtained from Dr A. De Lerma Barbaro, Clinical and Biological Sciences, University of Insubria, Varese, Italy |
| Reference paper | Science 1982;216:1233-1235 - DOI: 10.1126/science.6177045 - PMID: 6177045 |
| Morphology and growth | continuous culture, grown in suspension, morphology lymphoblast, round, large and single cells. Some giant cells |
| Culture conditions | RPMI 1640 + 10% FBS + 2mM L-Glutamine; Maintain cultures between 2-9 x 10^5 cells/ml; split 1:3-1:10; 37°C, 5% CO2 |
| Properties | Used in the study of erythroid cell differentiation and globin gene expression |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Freezing medium | 90% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. Multiplex PCR: tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers |
| Comments | Some cells weakly adherent |