ICLC logo Cell bank
IRCCS Ospedale Policlinico San Martino
Interlab Cell Line Collection (ICLC)
On-Line Catalogue

Home - Search by name - Free text search - Search by properties

Cell line nameA549
Accession numberICLC HTL03001
Brief descriptionSpecies: human, Caucasian; Tissue: lung; Tumor: carcinoma
DescriptionSpecies: human, Caucasian male 58 year old; Tissue: lung; Tumor: carcinoma
DepositorDr L. Lanfrancone, Experimental Oncology Dept, European Institute of Oncology, Milan, Italy
Reference paperJ Natl Cancer Inst 1973;51:1417-1423 - DOI: 10.1093/jnci/51.5.1417 - PMID: 4357758
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:4 using trypsin/EDTA; 37°C, 5% CO2
PropertiesThe cells could synthesize lecithin and are positive for keratin
DistributionCell line available for distribution. For non-commercial investigative use only
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Multiplex PCR: tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers
Further bibliographyInt J Cancer 1976;17:62-70 - DOI: 10.1002/ijc.2910170110 - PMID: 175022

For any info contact the Interlab Cell Line Collection
HTML by Paolo Romano