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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | A549 |
| Accession number | ICLC HTL03001 |
| Brief description | Species: human, Caucasian; Tissue: lung; Tumor: carcinoma |
| Description | Species: human, Caucasian male 58 year old; Tissue: lung; Tumor: carcinoma |
| Depositor | Dr L. Lanfrancone, Experimental Oncology Dept, European Institute of Oncology, Milan, Italy |
| Reference paper | J Natl Cancer Inst 1973;51:1417-1423 - DOI: 10.1093/jnci/51.5.1417 - PMID: 4357758 |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial |
| Culture conditions | DMEM + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:4 using trypsin/EDTA; 37°C, 5% CO2 |
| Properties | The cells could synthesize lecithin and are positive for keratin |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. Multiplex PCR: tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers |
| Further bibliography | Int J Cancer 1976;17:62-70 - DOI: 10.1002/ijc.2910170110
- PMID: 175022
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