Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell line name | Rj2.2.5 |
Accession number | ICLC HTL02002 |
Brief description | Species: human, Black; Tumor: lymphoma, Burkitt |
Description | Species: human, Black male 11 year old; Tumor: lymphoma, Burkitt; Derived from: Raji cell line |
Depositor | Dr A. De Lerma Barbaro, Clinical and Biological Sciences, University of Insubria, Varese, Italy. Established by R.S. Accolla in 1983. |
Reference paper | J Exp Med 1983;157:1053-8 - DOI: 10.1084/jem.157.3.1053 - PMID: 6403646 |
Morphology and growth | continuous culture, grown in suspension, morphology lymphoblast-like |
Culture conditions | RPMI 1640 + 10% FBS + 2mM L-Glutamine; split cultures 1:3-1:6; 37C, 5% CO2 |
Properties | HLA-ABC+; HLA-DR-,DQ-,DP- |
Distribution | Cell line available for distribution. For non-commercial investigative use only. The original paper must be cited in every publication |
Hazard | |
Freezing medium | 90% FBS + 10% DMSO |
Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |