Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell line name | SK-MEL-24 |
Accession number | ICLC HTL02001 |
Brief description | Species: human, Caucasian; Tissue: skin; Tumor: melanoma |
Description | Species: human, Caucasian male 67 year old; Tissue: skin; Tumor: melanoma; Derived from: lymph node metastasis of a malignant melanoma |
Depositor | Prof. S. Toma, Oncology, Biology and Genetic Dept, University of Genoa, Italy |
Reference paper | Unknown |
Morphology and growth | continuous culture, grown as monolayer, morphology stellate |
Culture conditions | MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids + 1mM Na pyruvate; split 1:3-1:5 using trypsin/EDTA (10-15 min); 37C, 5% CO2 |
Distribution | Cell line available for distribution. For non-commercial investigative use only |
Hazard | |
Passage number | 30 |
Tumorigenicity | tumorigenic in nude mice |
Freezing medium | Culture medium + 50% FBS + 10% DMSO |
Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. PCR analysis: confirmed as human with cytochrome c oxidase subunit I (cox I) primers |
Further bibliography | J Natl Cancer Inst 1977;58:209-214 - DOI: 10.1093/jnci/58.2.209
- PMID: 833871
J Natl Cancer Inst 1977;59:221-226 - DOI: 10.1093/jnci/59.1.221 - PMID: 327080 |