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Interlab Cell Line Collection (ICLC)
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Cell line nameSK-MEL-5
Accession numberICLC HTL01020
Brief descriptionSpecies: human, Caucasian; Tissue: skin; Tumor: melanoma
DescriptionSpecies: human, Caucasian female 24 year old; Tissue: skin; Tumor: melanoma; Derived from: axillary node
DepositorProf. S. Toma, Oncology, Biology and Genetic Dept, University of Genoa, Italy
Reference paperJ Natl Cancer Inst 1977;58:209-214 - DOI: 10.1093/jnci/58.2.209 - PMID: 833871
Morphology and growthcontinuous culture, grown as monolayer, morphology stellate
Culture conditionsMEM (EBSS) +10% FBS + 1mM Na pyruvate + 1% non-essential amino acids; split confluent cultures 1:3-1:6 using trypsin/EDTA; 37C, 5% CO2
DistributionCell line available for distribution. For non-commercial investigative use only
Tumorigenicitytumorigenic in nude mice
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
PCR analysis: confirmed as human with cytochrome c oxidase subunit I (cox I) primers
Further bibliographyJ Natl Cancer Inst 1977;59:221-226 - DOI: 10.1093/jnci/59.1.221 - PMID: 327080

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