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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | SK-MEL-5 |
| Accession number | ICLC HTL01020 |
| Brief description | Species: human, Caucasian; Tissue: skin; Tumor: melanoma |
| Description | Species: human, Caucasian female 24 year old; Tissue: skin; Tumor: melanoma; Derived from: axillary node |
| Depositor | Prof. S. Toma, Oncology, Biology and Genetic Dept, University of Genoa, Italy |
| Reference paper | J Natl Cancer Inst 1977;58:209-214 - DOI: 10.1093/jnci/58.2.209 - PMID: 833871 |
| Morphology and growth | continuous culture, grown as monolayer, morphology stellate |
| Culture conditions | MEM (EBSS) +10% FBS + 1mM Na pyruvate + 1% non-essential amino acids; split confluent cultures 1:3-1:6 using trypsin/EDTA; 37C, 5% CO2 |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Tumorigenicity | tumorigenic in nude mice |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. PCR analysis: confirmed as human with cytochrome c oxidase subunit I (cox I) primers |
| Further bibliography | J Natl Cancer Inst 1977;59:221-226 - DOI: 10.1093/jnci/59.1.221
- PMID: 327080
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