 |
Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
||
|
Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
||
| Cell line name | CFPAC-1 |
| Accession number | ICLC HTL01019 |
| Brief description | Species: human, Caucasian; Tissue: pancreas; Tumor: adenocarcinoma, ductal |
| Description | Species: human, Caucasian male 26 year old; Tissue: pancreas; Tumor: adenocarcinoma, ductal; Derived from: liver metastasis from a patient with cystic fibrosis |
| Depositor | Dr A. De Lerma Barbaro, Clinical and Biological Sciences, University of Insubria, Varese, Italy. |
| Reference paper | Proc Natl Acad Sci USA 1990;87:4012-4016 - DOI: 10.1073/pnas.87.10.4012 - PMID: 1692630 |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial |
| Culture conditions | Iscove's modified Dulbecco's medium + 10% FBS + 2mM L-Glutamine ; split confluent cultures 1:3-1:6 using trypsin/EDTA (20 min.) or scraper; 37C, 5% CO2 |
| Properties | CEA production |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Tumorigenicity | tumorigenic in nude mice |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. PCR analysis: confirmed as human with cytochrome c oxidase subunit I (cox I) primers |
| Comments | The cells are very difficult to detach and to disaggregate |