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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | MSTO-211H |
| Accession number | ICLC HTL01018 |
| Brief description | Species: human, Caucasian; Tumor: mesothelioma |
| Description | Species: human, Caucasian male 62 year old; Tumor: mesothelioma |
| Depositor | Dr L. Emionite, Preclinical Oncology Dept, CBA, Genoa, Italy |
| Reference paper | Differentiation 1988; 37:158-171 - DOI: 10.1111/j.1432-0436.1988.tb00806.x - PMID: 2840315 |
| Morphology and growth | continuous culture, grown as monolayer, morphology fibroblast-like |
| Culture conditions | RPMI 1640 + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:5-1:10 using trypsin/EDTA; 37C, 5% CO2 |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | Cell line of human origin. There is no evidence for the presence of infectious viruses or toxic products. However, it is recommended that cultures are handled under Biosafety Level 2 containment (Italian Decree Law 626/94). |
| Species validation | Validated by HLA-DP beta: confirmed as human by PCR |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |