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Cell line name5637
Accession numberICLC HTL01017
Brief descriptionSpecies: human, Caucasian; Tissue: bladder; Tumor: carcinoma
DescriptionSpecies: human, Caucasian male 68 year old; Tissue: bladder; Tumor: carcinoma
DepositorDr M. Musso, Oncology, Biology and Genetic Dept, Genoa, Italy
Reference paperNatl Cancer Inst Monogr 1978;49:5-9 - PMID: 571047
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial
Culture conditionsRPMI 1640 + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:4-1:8 using trypsin/EDTA (8-10 min.); 37C, 5% CO2
PropertiesGM-CSF, IL-1, IL-6 production
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by HLA-DP beta: confirmed as human by PCR
Tumorigenicitytumorigenic in nude mice
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Amelogenin: X,Y; vWA: 18; FGA: 22; TH01: 7,9; D18S51: --; D21S11: 36; D8S1179: 10,16
Further bibliographyJ Natl Cancer Inst 1977;59:221-226 - DOI: 10.1093/jnci/59.1.221 - PMID: 327080

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