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Interlab Cell Line Collection (ICLC)
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Cell line nameLS180
Accession numberICLC HTL01016
Brief descriptionSpecies: human, Caucasian; Tissue: colon; Tumor: adenocarcinoma, colorectal
DescriptionSpecies: human, Caucasian female 58 year old; Tissue: colon; Tumor: adenocarcinoma, colorectal
DepositorDr L. Varesco, Exp. Oncology Dept, IST, Genoa, Italy
Reference paperIn Vitro 1976;12:180-191 - DOI: 10.1007/BF02796440 - PMID: 1262041
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial
Culture conditionsMEM (EBSS) + 15% FBS + 2mM L-Glutamine + 1% non-essential amino acids; split cultures 1:4-1:6 using trypsin/EDTA (for at least 10 min.), or by scraping; 37C, 5% CO2
PropertiesCEA, IL-6, IL-10 and mucin production
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by HLA-DP beta: confirmed as human by PCR
Tumorigenicitytumorigenic in nude mice
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
CommentsThe cells are difficult to detach

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