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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell line name | MONO-MAC-6 |
Accession number | ICLC HTL01015 |
Brief description | Species: human; Tissue: peripheral blood; Tumor: leukemia, acute monocytic |
Description | Species: human male 64 year old; Tissue: peripheral blood; Tumor: leukemia, acute monocytic |
Depositor | Dr A. De Lerma Barbaro, Clinical and Biological Sciences, University of Insubria, Varese, Italy. |
Reference paper | Int J Cancer 1988;41:456-461 - DOI: 10.1002/ijc.2910410324 - PMID: 3162233 |
Morphology and growth | continuous culture, grown in suspension, morphology round, multiformed cells, some giant cells |
Culture conditions | RPMI 1640 + 10% FBS + 2mM L-Glutamine + 1mM Na pyruvate + 1% non-essential amino acids + 9 ug/ml bovine insulin ; maintain cultures between 3x10^5 and 1x10^6 cells/ml; split 1:3-1:6; 37C, 5% CO2 |
Distribution | Cell line available for distribution. For non-commercial investigative use only |
Hazard | |
Species validation | Validated by HLA-DP beta: confirmed as human by PCR |
Freezing medium | 90% FBS + 10% DMSO |
Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |