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Cell line nameGF-D8
Accession numberICLC HTL01009
Brief descriptionSpecies: human; Tissue: peripheral blood; Tumor: leukemia, acute myeloid
DescriptionSpecies: human male 82 year old; Tissue: peripheral blood; Tumor: leukemia, acute myeloid
DepositorDr A. Rambaldi, Divisione di Ematologia Ospedali Riuniti, Bergamo, Italy
Reference paperBlood 1993;81:1376-1383 - PMID: 8443395
Morphology and growthcontinuous culture, grown in suspension, morphology round, immature poorly differentiated myeloid blasts, some giant cells
Culture conditionsRPMI 1640 + 20% FBS + 2mM L-Glutamine + 10-50 ng/ml GM-CSF; split 1:2-1:3; 37C, 5% CO2
PropertiesGM-CSF and IL-3 dependent
DistributionCell line available for distribution, only as frozen ampoule. For non-commercial investigative use only
Freezing medium90% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
PCR analysis: confirmed as human with cytochrome c oxidase subunit I (cox I) primers
Further bibliographyGenes, Chromosomes Cancer 1999;24:213-221 - PMID: 10451701
Hematol Oncol 1995;13:177-183 - DOI: 10.1002/hon.2900130402 - PMID: 7557894
CommentsKaryotype: 47,X,der(Y)t(Y;12)del(Y),der(5;15)t(5;7)t(7;15),inv(7)x2,der(7)t(7;15)del(7)del(15),dup(8)+der(8)trp(8)ins(8;11)dup(11),der(11)dup(11)t(8;11)trp(8),der(11)t(11;17),der(12)t(7;12)del(12),+13,-15,-17

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