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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | IST-MES1 |
| Accession number | ICLC HTL01005 |
| Brief description | Species: human, Caucasian; Tissue: pleural effusion; Tumor: mesothelioma |
| Description | Species: human, Caucasian female 70 years old; Tissue: pleural effusion; Tumor: mesothelioma |
| Depositor | Dr A.M. Orengo, Immunopharmacology Dept, IST, Genoa, Italy |
| Reference paper | Eur Respir J 1999;13:527-534 - DOI: 10.1183/09031936.99.13352799 - PMID: 10232421 |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial-like |
| Culture conditions | DMEM + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Split confluent cultures 1:2-1:3 using trypsin/EDTA; 37C, 5% CO2 |
| Properties | IL-6 and TGF-beta2 production. Tag protein expression in the nucleus. CAM5.2+, HBME-1+ |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by HLA-DP beta: confirmed as human by PCR |
| Passage number | 54 |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | Int J Cancer 1998;78:740-749 - DOI: 10.1002/(sici)1097-0215(19981209)78:6<740::aid-ijc12>3.0.co;2-5
- PMID: 9833768
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