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Interlab Cell Line Collection (ICLC)
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Cell line nameIST-MES1
Accession numberICLC HTL01005
Brief descriptionSpecies: human, Caucasian; Tissue: pleural effusion; Tumor: mesothelioma
DescriptionSpecies: human, Caucasian female 70 years old; Tissue: pleural effusion; Tumor: mesothelioma
DepositorDr A.M. Orengo, Immunopharmacology Dept, IST, Genoa, Italy
Reference paperEur Respir J 1999;13:527-534 - DOI: 10.1183/09031936.99.13352799 - PMID: 10232421
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like
Culture conditionsDMEM + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Split confluent cultures 1:2-1:3 using trypsin/EDTA; 37C, 5% CO2
PropertiesIL-6 and TGF-beta2 production. Tag protein expression in the nucleus. CAM5.2+, HBME-1+
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by HLA-DP beta: confirmed as human by PCR
Passage number54
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyInt J Cancer 1998;78:740-749 - DOI: 10.1002/(sici)1097-0215(19981209)78:6<740::aid-ijc12>3.0.co;2-5 - PMID: 9833768

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