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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | 1301 |
| Accession number | ICLC HTL01004 |
| Brief description | Species: human, Caucasian; Tissue: blood; Tumor: leukemia, acute lymphoblastic T cell |
| Description | Species: human, Caucasian female 4 years old; Tissue: blood; Tumor: leukemia, acute lymphoblastic T cell; Derived from: CCRF-CEM |
| Depositor | DAKO S.p.A., Milan, Italy; DAKO A/S, Glostrup, Denmark |
| Reference paper | Dev Biol Stand 1979;42:193-197 - PMID: 223921 |
| Morphology and growth | continuous culture, grown in suspension, morphology lymphoblastic, cells growing single and in clusters |
| Culture conditions | RPMI 1640 + 10% FBS + 2mM L-Glutamine; maintain cultures between 2 and 9x10^5 cells/ml; split 1:5-1:10 every 2 to 3 days; 37C, 5% CO2 |
| Properties | The cell line has very long telomeres |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by HLA-DP beta: confirmed as human by PCR |
| Karyology | approx. tetraploid |
| Freezing medium | 90% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | Nucleic Acids Res. 1998;26(16):3651-6 - DOI: 10.1093/nar/26.16.3651
- PMID: 9685479
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