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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | ALL-PO |
| Accession number | ICLC HTL01003 |
| Brief description | Species: human; Tissue: bone marrow; Tumor: leukemia, acute lymphoblastic |
| Description | Species: human female 4 months old; Tissue: bone marrow; Tumor: leukemia, acute lymphoblastic |
| Depositor | Dr A. Biondi, Clinica Pediatrica, Universita' Milano Bicocca, Monza, Italy |
| Reference paper | Leuk Res 1997;21:1107-1114 - DOI: 10.1016/s0145-2126(97)00092-1 - PMID: 9444945 |
| Morphology and growth | continuous culture, grown in suspension, morphology lymphoblastic, round, single cells |
| Culture conditions | RPMI 1640 + 10% FBS; maintain cultures between 0.5-1 x 10^6 cells/ml; split 1:3-1:6; 37C, 5% CO2 |
| Distribution | Cell line available for distribution upon consent of the depositor. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by HLA-DP beta: confirmed as human by PCR |
| Karyology | t(4;11) translocation |
| Freezing medium | 90% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Comments | Thaw the cells with medium supplemented with 20% FBS. |