 |
Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
||
|
Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
||
| Cell line name | MPP 89 |
| Accession number | ICLC HTL00012 |
| Brief description | Species: human; Tissue: pleural effusion; Tumor: mesothelioma |
| Description | Species: human male 67 years old; Tissue: pleural effusion; Tumor: mesothelioma |
| Depositor | Dr P.G. Betta, Dr R. Libener, Ist. Anatomia Patologica, Alessandria, Italy |
| Reference paper | Eur Respir J 1999;13:527-534 - DOI: 10.1183/09031936.99.13352799 - PMID: 10232421 |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial-like |
| Culture conditions | Ham's F10 + 15% FBS + 2mM L-Glutamine; Split confluent cultures 1:3 twice a week using trypsin/EDTA; 37C, 5% CO2 |
| Properties | CAM5.2+; HBME-1+; CEA-; LeuM1-; Calretinina+; LeuM6-; LeuM3- |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by HLA-DP beta: confirmed as human by PCR |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |