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IRCCS Ospedale Policlinico San Martino
Interlab Cell Line Collection (ICLC)
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Cell line nameMPP 89
Accession numberICLC HTL00012
Brief descriptionSpecies: human; Tissue: pleural effusion; Tumor: mesothelioma
DescriptionSpecies: human male 67 years old; Tissue: pleural effusion; Tumor: mesothelioma
DepositorDr P.G. Betta, Dr R. Libener, Ist. Anatomia Patologica, Alessandria, Italy
Reference paperEur Respir J 1999;13:527-534 - DOI: 10.1183/09031936.99.13352799 - PMID: 10232421
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial-like
Culture conditionsHam's F10 + 15% FBS + 2mM L-Glutamine; Split confluent cultures 1:3 twice a week using trypsin/EDTA; 37C, 5% CO2
PropertiesCAM5.2+; HBME-1+; CEA-; LeuM1-; Calretinina+; LeuM6-; LeuM3-
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by HLA-DP beta: confirmed as human by PCR
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.

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