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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | BT-474 |
| Accession number | ICLC HTL00008 |
| Brief description | Species: human, Caucasian; Tissue: breast; Tumor: carcinoma, ductal |
| Description | Species: human, Caucasian female 60 years old; Tissue: breast; Tumor: carcinoma, ductal |
| Depositor | Dr L. Varesco, Exp. Oncology Dept, IST, Genoa, Italy |
| Reference paper | J Natl Cancer Inst 1978;61:967-978 - PMID: 212572 |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial, compact, multi-layered colonies, rarely become confluent |
| Culture conditions | RPMI 1640 + 10% FBS + 2mM L-Glutamine + 1mM Na pyruvate + 0,01 mg/ml Bovine insulin; split cultures 1:2-1:3 using trypsin/EDTA (about 10 min.); 37C, 5% CO2 |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Tumorigenicity | tumorigenic in nude mice |
| Freezing medium | 90% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | In Vitro 1979;15:723-729 - DOI: 10.1007/BF02618252
- PMID: 94035
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| Comments | The cells grow slowly |