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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | Hs 578T |
| Accession number | ICLC HTL00007 |
| Brief description | Species: human, Caucasian; Tissue: breast; Tumor: carcinoma |
| Description | Species: human, Caucasian female 74 years old; Tissue: breast; Tumor: carcinoma |
| Depositor | Dr L. Varesco, Exp. Oncology Dept, IST, Genoa, Italy |
| Reference paper | J Natl Cancer Inst 1977;58:1795-1806 - DOI: 10.1093/jnci/58.6.1795 - PMID: 864756 |
| Morphology and growth | continuous culture, grown as monolayer, morphology epithelial |
| Culture conditions | DMEM (4,5g/L glucose) + 10% FBS + 2mM L-Glutamine + 10ug/ml Bovine insulin; split cultures 1:2-5 using trypsin/EDTA; 37C, 5% CO2 |
| Properties | estrogen receptor not expressed |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by HLA-DP beta: confirmed as human by PCR |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Further bibliography | Cancer Res 1990;118:48 - PMID: 2152773
Cancer Res 1997;57:5386-5390 - PMID: 9393764 J Natl Cancer Inst 1979;62:225-230 - PMID: 283258 |