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Cell line nameHs 578T
Accession numberICLC HTL00007
Brief descriptionSpecies: human, Caucasian; Tissue: breast; Tumor: carcinoma
DescriptionSpecies: human, Caucasian female 74 years old; Tissue: breast; Tumor: carcinoma
DepositorDr L. Varesco, Exp. Oncology Dept, IST, Genoa, Italy
Reference paperJ Natl Cancer Inst 1977;58:1795-1806 - DOI: 10.1093/jnci/58.6.1795 - PMID: 864756
Morphology and growthcontinuous culture, grown as monolayer, morphology epithelial
Culture conditionsDMEM (4,5g/L glucose) + 10% FBS + 2mM L-Glutamine + 10ug/ml Bovine insulin; split cultures 1:2-5 using trypsin/EDTA; 37C, 5% CO2
Propertiesestrogen receptor not expressed
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by HLA-DP beta: confirmed as human by PCR
Freezing mediumCulture medium + 50% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
Further bibliographyCancer Res 1990;118:48 - PMID: 2152773
Cancer Res 1997;57:5386-5390 - PMID: 9393764
J Natl Cancer Inst 1979;62:225-230 - PMID: 283258

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