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Interlab Cell Line Collection (ICLC)
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Cell line namePF-382
Accession numberICLC HTL00006
Brief descriptionSpecies: human; Tissue: pleural effusion; Tumor: leukemia, T cell
DescriptionSpecies: human female 6 years old; Tissue: pleural effusion; Tumor: leukemia, T cell; Derived from: a patient with acute lymphoblastic leukemia (ALL) in second relapse
DepositorDr L. Pegoraro, Cytogenetics and Molecular Biology Dept, University of Turin, Italy
Reference paperJ Natl Cancer Inst 1985;75:285-290 - PMID: 2410652
Morphology and growthcontinuous culture, grown in suspension, morphology round and oval cells
Culture conditionsRPMI 1640 + 10-20% FBS + 2mM L-Glutamine; maintain cultures between 0.5-2 x 10^6 cells/ml; split 1:2-1:5; 37C, 5% CO2
DistributionCell line available for distribution. For non-commercial investigative use only
Species validationValidated by HLA-DP beta: confirmed as human by PCR
Freezing medium90% FBS + 10% DMSO
Validation assaysIdentification confirmed by Short Tandem Repeat (STR) profile.
CommentsThe cells should be frozen at not less than 5 x 10^6 cells/ampoule. The viability may be low after thawing; thaw with medium with 20% FBS

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