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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | PF-382 |
| Accession number | ICLC HTL00006 |
| Brief description | Species: human; Tissue: pleural effusion; Tumor: leukemia, T cell |
| Description | Species: human female 6 years old; Tissue: pleural effusion; Tumor: leukemia, T cell; Derived from: a patient with acute lymphoblastic leukemia (ALL) in second relapse |
| Depositor | Dr L. Pegoraro, Cytogenetics and Molecular Biology Dept, University of Turin, Italy |
| Reference paper | J Natl Cancer Inst 1985;75:285-290 - PMID: 2410652 |
| Morphology and growth | continuous culture, grown in suspension, morphology round and oval cells |
| Culture conditions | RPMI 1640 + 10-20% FBS + 2mM L-Glutamine; maintain cultures between 0.5-2 x 10^6 cells/ml; split 1:2-1:5; 37C, 5% CO2 |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by HLA-DP beta: confirmed as human by PCR |
| Freezing medium | 90% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Comments | The cells should be frozen at not less than 5 x 10^6 cells/ampoule. The viability may be low after thawing; thaw with medium with 20% FBS |