Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell line name | WiDr |
Accession number | ICLC HTL00003 |
Brief description | Species: human; Tissue: colon; Tumor: colorectal adenocarcinoma |
Description | Species: human female 44 years old; Tissue: colon; Tumor: colorectal adenocarcinoma |
Depositor | Dr L. Varesco, Exp. Oncology Dept, IST, Genoa, Italy |
Reference paper | Cancer Res 1974;34:105-108 - PMID: 4203458 |
Morphology and growth | continuous culture, grown as monolayer, morphology epithelial |
Culture conditions | MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; split confluent cultures 1:10-1:20 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2 |
Properties | Products: carcinoembryonic antigen (CEA), colon specific antigen (CSAp), transforming growth factor beta, keratin; epidermal growth factor (EGF) receptor and p53 antigen expression. The cells are negative for Colon Antigen 3 expression. HLA: A24, A32; B15, B18 |
Distribution | Cell line available for distribution. For non-commercial investigative use only |
Hazard | |
Species validation | Validated by HLA-DP beta: confirmed as human by PCR |
Tumorigenicity | tumorigenic in nude mice |
Freezing medium | Culture medium + 50% FBS + 10% DMSO |
Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
Further bibliography | In Vitro 1979;15:401-408 - DOI: 10.1007/BF02618407
- PMID: 90012
Proc Natl Acad Sci USA 1990;87:7555-7559 - DOI: 10.1073/pnas.87.19.7555 - PMID: 1699228 Science 1985;230:943-945 - DOI: 10.1126/science.3933111 - PMID: 3933111 |
Comments | Short tandem repeat (STR) genetic profiling has shown this cell line to be a derivative of HT-29 cell line. |