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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | RAJI |
| Accession number | ICLC HTL00002 |
| Brief description | Species: human, Black; Tumor: lymphoma, Burkitt |
| Description | Species: human, Black male 11 years old; Tumor: lymphoma, Burkitt |
| Depositor | Prof. S. Toma, Oncology, Biology and Genetic Dept, University of Genoa, Italy |
| Reference paper | Lancet 1964;1:238-240 |
| Morphology and growth | continuous culture, grown in suspension, morphology lymphoblast-like, round cells, single and in clumps |
| Culture conditions | RPMI 1640 + 10% FBS + 2mM L-Glutamine; maintain cultures between 0.4-1 x 10^6 cells/ml; split 1:5-1:10; 37C, 5% CO2 |
| Properties | EBNA positive |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by HLA-DP beta: confirmed as human by PCR |
| Freezing medium | 90% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. Amelogenin: X,Y; vWA: 16,19,18; FGA: 19,27; TH01: 6,7; D18S51: 17; D21S11: 28,31; D8S1179: 14,15 |
| Further bibliography | J Exp Med 1976;143:1528-1533 - DOI: 10.1084/jem.143.6.1528
- PMID: 1083890
J Natl Cancer Inst 1966;37:547 - PMID: 4288580 Trans N Y Acad Sci 1966;29:71 - DOI: 10.1111/j.2164-0947.1966.tb02253.x - PMID: 5232223 |
| Comments | After thawing the cells look suffering; thaw with medium supplemented with 20% FBS |