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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | HFFF2 |
| Accession number | ICLC HL95002 |
| Brief description | Species: human, Caucasian; Tissue: foreskin, fetal |
| Description | Species: human, Caucasian 14-18 weeks old; Tissue: foreskin, fetal |
| Depositor | Obtained from ECACC. |
| Reference paper | Unknown |
| Morphology and growth | continuous culture, grown as monolayer, morphology fibroblast |
| Culture conditions | DMEM + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:2-1:4 using trypsin/EDTA; seed at 2-3x104 cells/cm2; 37C, 5% CO2. The cells grow very slowly. |
| Properties | virology; Susceptible to: cytomegalovirus, herpes simplex |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as human with MD, MPI, NP |
| Validated by HLA-DP beta: confirmed as human by PCR | |
| Passage number | 14 |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |