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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | MRC-5 |
| Accession number | ICLC HL95001 |
| Brief description | Species: human; Tissue: lung, fetal |
| Description | Species: human male 14 weeks old; Tissue: lung, fetal |
| Depositor | Obtained from ECACC. |
| Reference paper | Nature 1970;227:168-170 - DOI: 10.1038/227168a0 - PMID: 4316953 |
| Morphology and growth | continuous culture, grown as monolayer, morphology fibroblast-like |
| Culture conditions | MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Split confluent cultures 1:4-1:6 using trypsin/EDTA; the cells are difficult to disaggregate after tripsinization; seed at 2-4x104 cells/cm2. 37C, 5% CO2. |
| Properties | vaccine development; senescence; virology; Susceptible to: poliovirus 1, herpes simplex, vesic. stomatitis (Indiana) |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as human with MD, NP |
| Passage number | 21 |
| Karyology | diploid |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. Amelogenin: X,Y; vWA: 15; FGA: 21,23; TH01: 8; D18S51: 15,21; D21S11: 31.2; D8S1179: 13 |
| Further bibliography | Nature 1972;238:26 - DOI: 10.1038/239026a0
- PMID: 4342691
Nature 1972;239:316 - DOI: 10.1016/0006-291x(75)90293-4 |