Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell line name | MRC-5 |
Accession number | ICLC HL95001 |
Brief description | Species: human; Tissue: lung, fetal |
Description | Species: human male 14 weeks old; Tissue: lung, fetal |
Depositor | Obtained from ECACC. |
Reference paper | Nature 1970;227:168-170 - DOI: 10.1038/227168a0 - PMID: 4316953 |
Morphology and growth | continuous culture, grown as monolayer, morphology fibroblast-like |
Culture conditions | MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Split confluent cultures 1:4-1:6 using trypsin/EDTA; the cells are difficult to disaggregate after tripsinization; seed at 2-4x104 cells/cm2. 37C, 5% CO2. |
Properties | vaccine development; senescence; virology; Susceptible to: poliovirus 1, herpes simplex, vesic. stomatitis (Indiana) |
Distribution | Cell line available for distribution. For non-commercial investigative use only |
Hazard | |
Species validation | Validated by isoenzymes: confirmed as human with MD, NP |
Passage number | 21 |
Karyology | diploid |
Freezing medium | Culture medium + 50% FBS + 10% DMSO |
Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. Amelogenin: X,Y; vWA: 15; FGA: 21,23; TH01: 8; D18S51: 15,21; D21S11: 31.2; D8S1179: 13 |
Further bibliography | Nature 1972;238:26 - DOI: 10.1038/239026a0
- PMID: 4342691
Nature 1972;239:316 - DOI: 10.1016/0006-291x(75)90293-4 |