Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell line name | HECV |
Accession number | ICLC HL01001 |
Brief description | Species: human, Caucasian; Tissue: umbilical cord |
Description | Species: human, Caucasian female; Tissue: umbilical cord |
Depositor | Dr G. Di Domenico, Biology and Cellular and Molecular Pathology Dept, Naples, Italy |
Reference paper | Unknown |
Morphology and growth | continuous culture, grown as monolayer, morphology endothelial |
Culture conditions | DMEM + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:5-1:10 using trypsin/EDTA; 37C, 5% CO2 |
Distribution | Cell line available for distribution. For non-commercial investigative use only |
Hazard | |
Species validation | Validated by HLA-DP beta: confirmed as human by PCR |
Passage number | 13 |
Freezing medium | Culture medium + 50% FBS + 10% DMSO |
Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |