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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | IST-EBV-TW6B |
| Accession number | ICLC EBV04002 |
| Brief description | Species: human, Caucasian; Tissue: lymphocyte, B; Transformed by: Epstein-Barr |
| Description | Species: human, Caucasian male 75 year old; Tissue: lymphocyte, B; Transformed by: Epstein-Barr; Derived from: peripheral blood |
| Depositor | Cell line established at ICLC. Sample provided by the Rome Twin Register |
| Reference paper | Unknown |
| Morphology and growth | continuous culture, grown in suspension, morphology lymphoblast, cells growing as single cells or in clusters |
| Culture conditions | RPMI 1640 + 20% FBS + 1% non-essential amino acids; Maintain cultures between 3-9 x 100.000 cells/ml; split 1:5-1:10; 37C, 5% CO2 |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | Handle as Biosafety Level 2 containment |
| Passage number | 3 |
| Freezing medium | 90% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Comments | Homozygous twin of IST-EBV-TW6A |