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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | IST-EBV-TW5B |
| Accession number | ICLC EBV03010 |
| Brief description | Species: human; Tissue: lymphocyte, B; Transformed by: Epstein-Barr |
| Description | Species: human male 77 year old; Tissue: lymphocyte, B; Transformed by: Epstein-Barr; Derived from: peripheral blood |
| Depositor | Cell line established at ICLC cell bank. Sample provided by the Rome Twin Register |
| Reference paper | Unknown |
| Morphology and growth | continuous culture, grown in suspension, morphology lymphoblast, cells growing as single cells or in clusters, some adherent |
| Culture conditions | RPMI 1640 + 20% FBS + 2mM L-Glutamine; Maintain cultures between 3-9 x 100.000 cells/ml; split 1:3-1:6; 37C, 5% CO2 |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | Handle as Biosafety Level 2 containment |
| Passage number | 6 |
| Freezing medium | 90% FBS + 10% DMSO |
| Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
| Comments | Dizygous twin of IST-EBV-TW5A |