Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell line name | IST-EBV-TW3.2 |
Accession number | ICLC EBV03006 |
Brief description | Species: human; Tissue: lymphocyte, B; Transformed by: Epstein-Barr |
Description | Species: human male 76 year old; Tissue: lymphocyte, B; Transformed by: Epstein-Barr; Derived from: peripheral blood |
Depositor | Cell line established at ICLC cell bank. Sample provided by the Rome Twin Register |
Reference paper | Unknown |
Morphology and growth | continuous culture, grown in suspension, morphology lymphoblast, cells growing as single cells or in clusters |
Culture conditions | RPMI 1640 + 20% FBS + 2mM L-Glutamine; Maintain cultures between 2-9 x 100.000 cells/ml; split 1:4-1:8; 37C, 5% CO2 |
Distribution | Cell line available for distribution. For non-commercial investigative use only |
Hazard | Handle as Biosafety Level 2 containment |
Passage number | 4 |
Freezing medium | 90% FBS + 10% DMSO |
Validation assays | Identification confirmed by Short Tandem Repeat (STR) profile. |
Comments | Homozygous twin of IST-EBV-TW3.1 |