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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
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Cell bank IRCCS Ospedale Policlinico San Martino |
Interlab Cell Line Collection (ICLC) On-Line Catalogue |
Home - Search by name - Free text search - Search by properties |
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| Cell line name | WOP |
| Accession number | ICLC ATL99011 |
| Brief description | Species: mouse; Tissue: embryo; Transformed by: polyoma virusreplication defective polyoma virus; Original line: 3T3 |
| Description | Species: mouse; Tissue: embryo; Transformed by: polyoma virusreplication defective polyoma virus; Original line: 3T3 |
| Depositor | Dr Ugo Borello, Hystology and Medical Embryology Dept, University of Rome La Sapienza, Italy |
| Reference paper | Unknown |
| Morphology and growth | continuous culture, grown as monolayer, morphology fibroblast-like |
| Culture conditions | DMEM + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:10-1:20 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2 |
| Distribution | Cell line available for distribution. For non-commercial investigative use only |
| Hazard | |
| Species validation | Validated by isoenzymes: confirmed as mouse with NP, PepB |
| Freezing medium | Culture medium + 50% FBS + 10% DMSO |
| Further bibliography | J Virol 1985;54:739-749 |